Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen detection, genotyping, and disease monitoring. The RNA-guided, RNA-targeting clustered regularly interspaced short palindromic repeats (CRISPR) effector Cas13a (previously known as C2c2) exhibits a "collateral effect" of promiscuous ribonuclease activity upon target recognition. We combine the collateral effect of Cas13a with isothermal amplification to establish […]
Gootenberg, J. S., Abudayyeh, O. O., Lee, J. W., Essletzbichler, P., Dy, A. J., Joung, J., Verdine, V., Donghia, N., Daringer, N. M., Freije, C. A., Myhrvold, C., Bhattacharyya, R. P., Livny, J., Regev, A., Koonin, E. V., Hung, D. T., Sabeti, P. C., Collins, J. J., Zhang, F.
Serotonergic neurons project their axons pervasively throughout the brain and innervate various target fields in a space-filling manner, leading to tiled arrangements of their axon terminals to allow optimal allocation of serotonin among target neurons. Here we show that conditional deletion of the mouse protocadherin α (Pcdhα) gene cluster in serotonergic neurons disrupts local axonal […]
Chen, W. V., Nwakeze, C. L., Denny, C. A., OKeeffe, S., Rieger, M. A., Mountoufaris, G., Kirner, A., Dougherty, J. D., Hen, R., Wu, Q., Maniatis, T.
Meet the science marchersApril 27, 2017 Science News Staff, Wade, L., Matacic, C., Gibbons, A., Clery, D., Malakoff, D., Wadman, M., Barone, L. T., Stokstad, E., Escobar, H., Derworiz, C., Servick, K., Enserink, M.